KMID : 0379520000160010039
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Çѱ¹µ¶¼ºÇÐȸÁö 2000 Volume.16 No. 1 p.39 ~ p.45
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Antitoxic Effects of Palsun Brewing Water against Bacterial Endotoxin and Cadmium Induced Cytotoxicity
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Han Du-Seok
Kim Jin-Sun Han Jong-Hyun Lee Ho-Sub Kim Ji-Ju Kang Kil-Ung Baek Seung-Hwa
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Abstract
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This study was carried out to evaluate antitoxic effects Palsun Brewing Water against cadmium by colorimetric methods. The antitoxic activity of Palsun Brewing Water in NIH-3T3 fibroblasts was evaluated by MTT ({3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazoliumbromide} and SRB (sulforhodamine B protein) assays. The light microcopic study was carried out to observe morphological changes of the treated cells. These results were obtained as follows: The concentration of 10-2 mg/ml of Palsun Brewing Water was shown significant antitoxic activity against E. coli endotoxin and Salmonella endotoxin. The number of NIH-3T3 fibroblasts were antitoxin and tend to regenerate. These results suggest that Palsun Brewing Water retains a potential antitoxic activity.
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KEYWORD
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Antitoxic effect, Palsun Brewing Water, MTT and SRB assays
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